Use of encapsulated exogenous enzymes in the ruminant feed industry

Satish V Dakore

Livestock Development Officer Govt. of Maharashtra

The Ruminants i.e. cattle, sheep, and goats are specially adapted to digest high-fibre diet, such as grasses and other forages up to 40-100% of the diet. But the most of the fibre portion still may pass through GIT without being digested. It indicates the potential use of nutrients and it utilizing efficiency reduces. In ruminants feed cost accounting for up to 70% of production costs. As the production increases with less feed costs and enhance livestock economics stability by boosting the feed utilization efficiency. Moreover, the strategies for nutrient uses including nutritional management practices are to adding fibre degrading enzymes to animal feed. Inclusion of fiber digestive enzyme in the feed to smoother the digestion of residual fiber in lower gut is one of the planned.

            Exogenous enzymes are used to degrade complex fibrous feed materials and improve nutrient utilization and animal production responses. Although ruminants have a natural ability to digest fibrous feedstuffs through the action of microbial enzymes in the rumen, structural polysaccharides such as cellulose and hemicellulose, along with lignin-associated components, are not completely degraded. Consequently, a considerable proportion of the nutrients remains inaccessible to ruminal microorganisms.So, supplementation with exogenous enzymes can target specific substrates and enhance the degradation of fibrous feed components, thereby improving the efficiency of feed utilization. These enzymes may also accelerate the decomposition of complex feed materials, reduce nutrient losses and minimize feed wastage. Furthermore, exogenous enzyme supplementation can improve the nutritive value and digestibility of feeds and may help reduce the effects of certain anti-nutritional factors present in unconventional feed resources. Thus, the use of exogenous enzymes represents a promising nutritional strategy for enhancing feed utilization and improving animal performance.

Exogenous enzymes are enzymes produced outside the animal and added to feed to improve nutrient digestion, feed efficiency, and animal performance. Common examples include cellulases, xylanases, β-glucanases, amylases, proteases, phytases, and pectinases.

The potential benefits of using encapsulated fibrinolytic exogenous enzymesin animal feeds are;

  1. To breakdown anti-nutritional factors
  2. To increase feed digestibility, nutrient utilization and feed conversion efficiency
  3. To significant potential for improving feedutilization efficiency in ruminants
  4. To enhance the utilization of agricultural by-products leads to Potential reduction in feed costs
  5. To Improved animal productivity, as well as Potential reduction in nutrient losses and environmental footprint

Why encapsulated enzyme technologies needs

In ruminants, the situation is more complex because feed first enters the rumen, where a large microbial population naturally produces many digestive enzymes. Therefore, encapsulation techniques are essential for the enhancing stability and controlled release of enzyme as well as toprotect exogenous enzymes from degradation or inactivation in the rumen and deliver them more effectively to their intended site of action. Moreover, Protect enzymes from temperature, moisture, oxygen and pH fluctuations during feed processing and storage. It improves enzyme stability, shelf life along with improve interaction between the enzyme and feed particles as well as increase responses in animal performance.However, responses are not always consistent because enzyme effectiveness depends on enzyme type, dose, diet composition, encapsulation technology, rumen conditions, and animal characteristics.

Methods of encapsulation: techniques can be broadly classified into three groups

  1. physicochemical methods, such as thermal gelation, and emulsification
  2.  chemical methods, including polymerization and interfacial polycondensation
  3.  physical (mechanical) methods, such as solvent evaporation, spray drying, fluidized-bed coating, centrifugation, and extrusion

Application of encapsulated enzymes in animal feed:

To delivered the feed substance at targeted site of GIT, without degradation in the ruminal tract. Mask undesirable coating of nutrient to ensuring the stability of product for long periods of time as well as enhance bioavailability of product to improve animal performance of particular animal. Many studies have shown that encapsulation able to preserving the nutrient integrity during ruminal passage. Like, coating soybean meal with zein or wheat gluten improved its protection against ruminal degradationas compared to unprotected soyabean meal. The field has explored diverse approaches to microencapsulationcan function as a rumen-protection and controlled-release technology.This can help improve the nutrient efficiency, bioavailability, and biological effectiveness of dietary supplements in ruminants.Overall, potential use of microencapsulation strategy for delivering bioactive compounds to specific regions of the ruminant gastrointestinal tract. Despite the inherent complexities associated with its application, these studies collectively enhance our understanding of the feasibility and efficacy of microencapsulation for targeted nutrient delivery, with promising implications for improving nutrient utilization, animal performance, and overall well-being.

Conclusion and futures perspective

Low feed utilization efficiency remains a major challenge in livestock production, particularly in ruminants depends on forage-based diets. Several strategies, including selective breeding for improved feed efficiency and supplementation with exogenous microorganisms and fibre-degrading enzymes. Among these approaches, encapsulated exogenous enzyme supplementation prominent feed additive technology to enhance fibre digestibility in both the rumen and post-ruminal gastrointestinal tract. Future research should focus on developing encapsulated systems that provide controlled, site-specific release of enzymes within the gastrointestinal tract. Moreover, in vivo studies are essential to determine the bioavailability or efficacy of encapsulated enzymes and their effects on fibre digestion, nutrient utilization in ruminants. Collectively, these promising encapsulated technologies for enzyme delivery in ruminants for improving fibre digestion, feed utilization and economic potentiality